Jobs & opportunities

Vacancy for a PhD student (4 year)

The role of Joubert syndrome-associated proteins in neuronal development


PhD position is available in the framework of the Marie Curie Innovative Training Network (ITN), SCiLS.


Joubert syndrome (JBTS) is a neuronal ciliopathy, characterized by a distinctive brain malformation known as the "molar tooth sign", as well as heterogenous other manifestations both in the brain and other organs. The common mechanism of disease in the brain remains unclear, but is suggested to be loss of neural signalling. We developed an in vitro model to investigate neuronal development, by differentiating hiPSCs into excitatory neurons (iNeurons) and forebrain organoids. ESR8 will use three selected JBTS patient-derived hiPSCs from differentially localized modules (RPGRIP1L, ARMC9, and TMEM), as well as isogenic CRIPSR/Cas9-edited lines for neuronal network measurements using microelectrode arrays (MEAs). As iNeurons exhibit patterns of synchronized and rhythmic activity on MEAs, ESR8 will generate a developmental network MEA fingerprint that functionally describes the properties of the neural signalling circuits. Complemented with superresolution (STORM and CLEM, in collaboration with PO2-MPI-CBG) and live cell imaging of JBTS-associated markers, measurements of cilium formation/stability in organoids, and ciliary


Applicants should submit:


a personal statement explaining their motivation and project interests and the contact details of 2 academic referees


a CV (download at www.scils.eu; other formats  NOT accepted)


a form indicating the preference for one or more specific partner institute(s) and/or research project(s) (download preference form)


Please send these documents via email to simone.dusseljee@radboudumc.nl


Do NOT include other documents like diplomas.


PLEASE NOTE that applications that do not meet these requirements WILL NOT BE CONSIDERED.

Deadline

February 29, 2020, after which we will review applications and interview candidates.

Do NOT include other documents like diplomas.

PLEASE NOTE that applications that do not meet these requirements WILL NOT BE CONSIDERED.